Clone Selection


 

Select The Best Clones Using ValitaPREDICT & Cut Productivity Assessment  By Up To 50%

ValitaPREDICT applies ChemStress Fingerprinting to early quantitative assessment of a clones critical quality attributes (CQA's) to shorten productivity assessment timelines.

Have greater confidence in early stage clone selection using ChemStress Fingerprinting. The ValitaPREDICT assay, which allows early quantitative assessment of a clone’s Critical Quality Attribute profile (CQA), can be used for early clone selection during the cell line development process. ValitaPREDICT, using modelling strategies based on Clone qP, has been shown to:

  • Select the best clones to take to the mini-bioreactor stage with 90% accuracy;
  • Predict the mini-bioreactor performance with a R2 of 0.8.

Current methods to pick the best cell clone incorporate some variation of fed-batch, minibioreactor and product titre estimation. We have demonstrated that ValitaPREDICT can be used successfully:

  • In combination with microbioreactor to extend their reach and improve their efficiency;
  • In combination with fed-batch to bypass microbioreactor stage of screening;
  • In isolation to produce priority ranking to determine which clones go into the next stage of the selection process:
  • To widen the population of clones that can be screened.

For more information, download the poster: Microplate Based Stress Resistance Fingerprinting Technology for Rapid, High-throughput Cell Line Development.

 

Lower Cost

Shortening of drug development time;
Significantly reduced consumable and cell culture costs;

 

Faster Results

Pick the best clone in up to 50% of the time Lower test cost and lower capital costs than existing technologies;

Greater Confidence 

Increased process knowledge of clones


 

technology Platform: ChemStress Fingerprinting

The ChemStress plate simulates the biological and environmental stresses a cell clone encounters in a bioreactor. Each of the wells of the ChemStress 96-well plate are pre-coated with multiple small molecule chemicals, which re-create the environmental and biological stresses of a bioreactor. Each chemical has been carefully selected to challenge a particular aspect of a cell clones biochemistry. Some of these challenges include: Nutrient Starvation; Oxidative Stress; pH, Osmolarity and Toxicity. For example, Brefeldin A inhibits protein transport and is a lactone antibiotic.

How To Generate A ChemStress Fingerprint

An individual cell clone is grown in the ChemStress plate for 3 days. After exposure to the chemical stressors, clonal growth and product titre are measured in response to each of the chemical stressors. This is read on a plate reader and analysed using the Valitacell software 'ValitaAPP'. ValitaAPP performs the analysis of a cell clones growth and titre profile using product-specific algorithms. This makes up a clones ChemStresss Fingerprint, and this fingerprint is unique to an individual cell clone or a media batch. 

1) An individual cell clone is grown in the ChemStress plate for 3 days and exposed to the numerous small molecule chemicals 2) A clones growth and productivity are measured on a plate reader 3) ValitaAPP software applies product-specific algorithms to analyse the growth and titre data 4) A PREDICT ChemStress fingerprint is generated to determine how stable a clone will be in the bioreactor.