Cell Profiling · Predict Stability · Confirm Identity · Control Media Lot Variation
At Valitacell, we recognise the value that exists in process control of cell based manufacturing systems. That's why we've developed ChemStress Fingerprinting - a novel platform technology which characterises cells using a panel of specific, small molecule chemical stressors and combines this with deep learning and AI to achieve the highest levels of bioprocess performance.
The Valita®STABILITY assay allows for rapid, low cost stability assessment using ChemStress Fingerprinting. In trials at industry sites we have demonstrated that Valita®STABILITY can be used to predict a clones stability status, and thus deliver significant benefits over existing technologies.
The Valita®QC assay applies ChemStress Fingerprinting for rapid quality control testing of media batches. Valita®QC can be used to detect poor quality media batches in comparison with an accepted reference lot. Valita®QC works on the basis that a cell clone and media combination has a highly unique stress response fingerprint, and any change in the fingerprint is due to changes in media formulation.
The Valita®PROFILE assay applies ChemStress Fingerprinting to rank clones in a population which allows users to rapidly and easily shortlist clones based on moderate and extreme behaviour. Valita®PROFILE is used to identify ‘extreme’ clones likely to struggle to maintain performance over a long period of time.
The Valita®ID assay allows users to uniquely identify cell lines with resolution down to the clonal level, to reliably discriminate & indicate instability when a clone becomes non-self. Valita®ID enables rapid traceability while at the same time providing the highest levels of quality assurance.
For more information on the ChemStress Fingerprinting technology, click here >>
ChemStress® Fingerprinting is an information-rich, analytical assay supplying data on the functional quality of clones, facilitating the selection of stable producer cells and high-quality media environments through the generation of cellular responses to chemical stressors. The technology draws on the unique biological signatures (e.g. growth and titer for CHO cells producing monoclonal antibodies) a given cell generates when it is grown in the presence of small molecule stressors, which have been selected to simulate toxic environments or stimulate specific stress response pathways. This data gives biological information on how specific clones respond to stressors, and whether these clones have the ability to remain stable and productive in these conditions. These cellular responses are unique identifiers, or ‘fingerprints’. While classic fingerprints are static measurements of identity, these ‘ChemStress® fingerprints’ are markers which, much like cells, can change overtime as a result of e.g. epigenetic silencing events, or loss of recombinant protein expression, giving early indications of loss of specific functionally. The diverse range of responses is a unique way of:
identifying cell populations
indicating divergence from its original state
indicating the growth quality of cell culture conditions.
ChemStress® employs the use of a novel 96-well microtiter plate, wherein each well is coated with a single specific chemical, selected based on its published ability to simulate specific conditions, or effect specific cellular pathways.