Technology Platform: ChemStress® Fingerprinting


ChemStress® Fingerprinting is an information-rich, analytical assay supplying data on the functional quality of clones, facilitating the selection of stable producer cells and high-quality media environments through the generation of cellular responses to chemical stressors. The technology draws on the unique biological signatures (e.g. growth and titer for CHO cells producing monoclonal antibodies) a given cell generates when it is grown in the presence of small molecule stressors, which have been selected to simulate toxic environments or stimulate specific stress response pathways. This data gives biological information on how specific clones respond to stressors, and whether these clones have the ability to remain stable and productive in these conditions. These cellular responses are unique identifiers, or ‘fingerprints’. While classic fingerprints are static measurements of identity, these ‘ChemStress® fingerprints’ are markers which, much like cells, can change overtime as a result of e.g. epigenetic silencing events, or loss of recombinant protein expression, giving early indications of loss of specific functionally. The diverse range of responses is a unique way of:

  • identifying cell populations

  • indicating divergence from its original state

  • indicating the growth quality of cell culture conditions. ChemStress® employs the use of a novel 96-well microtiter plate, wherein each well is coated with a single specific chemical, selected based on its published ability to simulate specific conditions, or effect specific cellular pathways.

 

Process Control with ChemStress Fingerprinting

How To Generate A ChemStress Fingerprint

An individual cell clone is grown in the ChemStress plate for 3 days. After exposure to the chemical stressors, clonal growth and product titre are measured in response to each of the chemical stressors. This is read on a plate reader and analysed using the Valitacell software 'ValitaAPP'. ValitaAPP performs the analysis of a cell clones growth and titre profile using product-specific algorithms. This makes up a clones ChemStresss Fingerprint, and this fingerprint is unique to an individual cell clone or a media batch. 

 

1) An individual cell clone is grown in the ChemStress plate for 3 days and exposed to the numerous small molecule chemicals 2) A clones growth and productivity are measured on a plate reader 3) ValitaAPP software applies product-specific algorithms to analyse the growth and titre data and a ChemStress fingerprint is generated.