Technology Platform: ChemStress Fingerprinting
The ChemStress plate simulates the biological and environmental stresses a cell clone encounters in a bioreactor. Each of the wells of the ChemStress 96-well plate are pre-coated with multiple small molecule chemicals, which re-create the environmental and biological stresses of a bioreactor. Each chemical has been carefully selected to challenge a particular aspect of a cell clones biochemistry. Some of these challenges include: Nutrient Starvation; Oxidative Stress; pH, Osmolarity and Toxicity. For example, Brefeldin A inhibits protein transport and is a lactone antibiotic.
Process Control with ChemStress
How To Generate A ChemStress Fingerprint
An individual cell clone is grown in the ChemStress plate for 3 days. After exposure to the chemical stressors, clonal growth and product titre are measured in response to each of the chemical stressors. This is read on a plate reader and analysed using the Valitacell software 'ValitaAPP'. ValitaAPP performs the analysis of a cell clones growth and titre profile using product-specific algorithms. This makes up a clones ChemStresss Fingerprint, and this fingerprint is unique to an individual cell clone or a media batch.
The ChemStress fingerprint can be used at various different stages during cell line development to shorten the drug production timeline by months. ChemStress fingerprinting has four product-specific applications;